ANALYTICAL MODEL FOR EXTRACTING INTRINSIC FLUORESCENCE IN TURBID MEDIA

被引:230
作者
WU, J
FELD, MS
RAVA, RP
机构
[1] George R Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA
来源
APPLIED OPTICS | 1993年 / 32卷 / 19期
关键词
FLUORESCENCE; DIFFUSE REFLECTANCE;
D O I
10.1364/AO.32.003585
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
In this paper we describe a photon migration approach for modeling fluorescence in an optically thick, turbid medium such as human tissue. In such a medium the intrinsic fluorescence spectrum of the fluorophores phi can be distorted by the interplay of many factors, including scattering and absorption, excitation and collection geometries, and boundary conditions. The model provides an analytical relationship between the bulk fluorescence spectrum F and the diffuse reflectance spectrum R for arbitrary geometries and boundary conditions. We demonstrate that the distortion can be simply and accurately removed by measuring R from the optically thick medium over the same wavelength range and in the same manner as F. Over a wide range of tissue parameters this relationship may be written as phi is-proportional-to F/R(eff), with R(eff) a corrected form of the measured diffuse reflectance. The validity of this approach is demonstrated in both laboratory experiments on human aortic media and by comparison with Monte Carlo simulations and diffusion theory. Connection with a previous algorithm for extracting intrinsic fluorescence is also discussed.
引用
收藏
页码:3585 / 3595
页数:11
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