SEPARATION OF ADENOVIRUS TYPE 4 COMPONENTS

The following procedure, resulting in separation of 6 different adenoverus type 4 viral components, all of which were identified in the electron microscope in their purified state, is proposed. The 1st stage comprised differential centrifugation in a discontinuous CsCl gradient of crude virus material which was followed by repeated isopycnic banding of the "non-soluble" components, thus producing a purified intact virus and empty capsids. In the 2nd stage all the soluble components were at least partially separated by anionic exchange chromatography. They eluted in the following sequences: fibers, hexons, pentons and soluble complete HA [hemagglutination]. In the subsequent stage zonal centrifugation was applied to obtain purified hexons and soluble complete HA and application of gelfiltration was needed to purify pentons from contaminating hexons.