PRODUCTION OF ANTIBODIES TO VITAMIN-B12

被引：13

作者：

AHRENSTEDT, SS ^{[1
]}

THORELL, JI ^{[1
]}

机构：

[1] UNIV LUND,MALMO GEN HOSP,DEPT NUCL MED,S-21401 MALMO,SWEDEN

来源：

CLINICA CHIMICA ACTA | 1979年 / 95卷 / 03期

关键词：

D O I：

10.1016/0009-8981(79)90191-8

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The conjugation of vitamin B-12 to HSA by means of a combined ethylcar-bodiimide N-hydroxysuccinimide conjugation method at pH 5.5 is described. It gave a ratio of 2.7 mol vitamin B-12/mol HSA. Five rabbits were immunized with this conjugate emulsified in Freund's complete adjuvant. After one booster injection high titre antisera (1 : 450 000-1 : 1 500 000) to vitamin B-12 were collected. © 1979.

引用

页码：419 / 423

页数：5

共 5 条

[1]

ALLEN RH, 1972, J BIOL CHEM, V247, P7695

[2]

ENDRES DB, 1978, CLIN CHEM, V24, P460

[3] PRODUCTION AND SPECIFICITY OF ANTIBODIES TO B12 DERIVATIVES [J].

GERSHMAN, H ;

ABELES, RH ;

NATHANSON, N ;

LEVINE, L .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1972, 153 (01) :407-+

[4] MEASUREMENT OF SERUM VITAMIN B12 LEVEL USING RASIOISOTOPE DILUTION AND COATED CHARCOAL [J].

LAU, KS ;

GOTTLIEB, C ;

WASSERMA.LR ;

HERBERT, V .

BLOOD-THE JOURNAL OF HEMATOLOGY, 1965, 26 (02) :202-&

[5] PRODUCTION AND PURIFICATION OF ANTIBODY AGAINST PROTEIN-VITAMIN-B12 CONJUGATES FOR RADIOIMMUNOASSAY PURPOSES [J].

VANDEWIEL, DF ;

GOEDEMANS, WT ;

WOLDRING, MG .

CLINICA CHIMICA ACTA, 1974, 56 (02) :143-149

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