DETECTION OF HERPESVIRUS TYPE-SPECIFIC ANTIBODY BY A MICRO SOLID-PHASE RADIOIMMUNOMETRIC ASSAY

被引：32

作者：

DREESMAN, GR

MATSON, DO

COURTNEY, RJ

ADAM, E

MELNICK, JL

机构：

[1] Department of Virology and Epidemiology, Baylor College of Medicine, Houston, TX

[2] Department of Microbiology, University of Tennessee, Knoxville, TN

来源：

INTERVIROLOGY | 1979年 / 12卷 / 02期

关键词：

Herpesvirus type-specific antibody; Radioimmunoassay for herpesvirus antibody; Type-specific herpesvirus glycoproteins;

D O I：

10.1159/000149077

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The testis performed by coating wells of microtiter plates with purified HSV-specified glycoproteins, VP123 and VP119, derived from HSV-1- and HSV-2-infected cells, respectively. The coated wells are then incubated with diluted unadsorbed sera and subsequently with radio-iodinated goat anti-human IgG reagent. Because these major viral envelope glycoproteins contain type-specific antigenic determinants, type-specific antibodies are preferentially detected in our assay. © 1979 S. Karger AG, Basel.

引用

页码：115 / 119

页数：5

共 18 条

[1]

ADAM E, 1973, AM J EPIDEMIOL, V96, P427

[2] ISOLATION AND CHARACTERIZATION OF A LARGE MOLECULAR-WEIGHT POLYPEPTIDE OF HERPES-SIMPLEX VIRUS TYPE-1 [J].