ENZYMATIC 2-STEP SYNTHESIS OF N-ACETYLNEURAMINIC ACID IN THE ENZYME MEMBRANE REACTOR

被引：137

作者：

KRAGL, U

GYGAX, D

GHISALBA, O

WANDREY, C

机构：

[1] FORSCHUNGSZENTRUM JULICH,INST BIOTECHNOL,POSTFACH 1913,W-5170 JULICH,GERMANY

[2] CIBA GEIGY AG,ZENT FORSCH LAB,CH-4002 BASEL,SWITZERLAND

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH | 1991年 / 30卷 / 07期

关键词：

D O I：

10.1002/anie.199108271

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The use of N-acetylglucosamine 2-epimerase from pig's liver together with N-acetylneuraminic aldolase leads to a particularly simple and effective process for the synthesis of N-acetylglucosamine (1) and alpha-pyruvic acid (3). The enzymes in homogeneous solution in the enzyme-membrane reactor catalyze the synthesis of N-acetylneuraminic acid (4) via 2 in a continuous process. The space-time yield is 109 gL-1 d-1.

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页码：827 / 828

页数：2

相关论文

共 17 条

[1] THE USE OF IMMOBILIZED GLYCOSYLTRANSFERASES IN THE SYNTHESIS OF SIALYLOLIGOSACCHARIDES [J].

AUGE, C ;

FERNANDEZFERNANDEZ, R ;

GAUTHERON, C .

CARBOHYDRATE RESEARCH, 1990, 200 :257-268

[2] SIALYL ALDOLASE IN ORGANIC-SYNTHESIS - FROM THE TROUT EGG ACID, 3-DEOXY-D-GLYCERO-D-GALACTO-2-NONULOSONIC ACID (KDN), TO BRANCHED-CHAIN HIGHER KETOSES AS POSSIBLE NEW CHIRONS [J].

AUGE, C ;

GAUTHERON, C ;

DAVID, S ;

MALLERON, A ;

CAVAYE, B ;

BOUXOM, B .

TETRAHEDRON, 1990, 46 (01) :201-214

[3] CELL-SURFACE CARBOHYDRATES AND THEIR INTERACTIONS .1. NMR OF N-ACETYL NEURAMINIC ACID [J].

BROWN, EB ;

BREY, WS ;

WELTNER, W .

BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 399 (01) :124-130

[4] COMPOSITION AND ENZYMATIC SYNTHESIS OF N-ACETYLNEURAMINIC ACID (SIALIC ACID) [J].

COMB, DG ;

ROSEMAN, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1958, 80 (02) :497-499

[5] REGULATORY ROLE OF ADENOSINE TRIPHOSPHATE ON HOG KIDNEY N-ACEYL-D-GLUCOSAMINE 2-EPIMERASE [J].

DATTA, A .

BIOCHEMISTRY, 1970, 9 (17) :3363-&

[6]

GOSH S, 1965, J BIOL CHEM, V240, P1531

[7] ENZYMES IN CARBOHYDRATE SYNTHESIS - N-ACETYLNEURAMINIC ACID ALDOLASE CATALYZED-REACTIONS AND PREPARATION OF N-ACETYL-2-DEOXY-D-NEURAMINIC ACID-DERIVATIVES [J].

KIM, MJ ;

HENNEN, WJ ;

SWEERS, HM ;

WONG, CH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (19) :6481-6486

[8]

KULA MR, 1987, METHOD ENZYMOL, V136, P9

[9] INHIBITION OF NEURAMINIDASE ACTIVITY BY DERIVATIVES OF 2-DEOXY-2,3-DEHYDRO-N-ACETYLNEURAMINIC ACID [J].

MEINDL, P ;

BODO, G ;

PALESE, P ;

SCHULMAN, J ;

TUPPY, H .

VIROLOGY, 1974, 58 (02) :457-463

[10] ENZYMIC-SYNTHESIS OF DI-SACCHARIDE AND TRI-SACCHARIDE GLYCOSIDES, USING GLYCOSIDASES AND BETA-D-GALACTOSIDE 3-ALPHA-SIALYL-TRANSFERASE [J].

NILSSON, KGI .

CARBOHYDRATE RESEARCH, 1989, 188 :9-17