THE IN-SITU SIZE OF THE DOPAMINE TRANSPORTER IS A TETRAMER AS ESTIMATED BY RADIATION INACTIVATION

被引：45

作者：

MILNER, HE

BELIVEAU, R

JARVIS, SM

机构：

[1] UNIV KENT, RES SCH BIOSCI, CANTERBURY CT2 7NJ, KENT, ENGLAND

[2] UNIV QUEBEC, DEPT CHIM, MONTREAL H3C 3P8, PQ, CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1190卷 / 01期

关键词：

DOPAMINE TRANSPORTER; RADIATION INACTIVATION; GBR-12935; (CANINE BRAIN MEMBRANE);

D O I：

10.1016/0005-2736(94)90051-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Radiation inactivation analysis of the mazindol-sensitive binding of the dopamine transporter inhibitor, [H-3]GBR-12935 to canine striatal membranes yielded a radiation inactivation target size of 278 +/- 16 kDa. This result, in conjunction with other findings in the literature demonstrating that the molecular mass of the dopamine transporter is similar to 70 kDa, suggests that the native form of the dopamine carrier in the neuronal membranes is a tetrameric assembly of identical 70 kDa subunits.

引用

页码：185 / 187

页数：3

共 17 条

[1]

Amara S G, 1991, Curr Opin Neurobiol, V1, P84, DOI 10.1016/0959-4388(91)90014-X

[2] THE DOPAMINE UPTAKE INHIBITOR GBR 12909 - SELECTIVITY AND MOLECULAR MECHANISM OF ACTION [J].