NUCLEAR DIVISION IN ISOLATED PROTOPLASTS FROM CELLS OF HIGHER PLANTS GROWN IN VITRO

被引：62

作者：

ERIKSSON, T

JONASSON, K

机构：

[1] Institute of Physiological Botany, University of Uppsala

来源：

PLANTA | 1969年 / 89卷 / 01期

关键词：

D O I：

10.1007/BF00386499

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Protoplasts were isolated from cell suspensions of Haplopappus gracilis. The cell walls were degraded by the cellulase preparation Onozuka P 1500" at a concentration of 5%. Sorbitol was found to work well as osmotic stabilizer in concentrations of 0.4-0.6 M. The protoplasts were cultured in growth medium after isolation; 3-5% went through nuclear division once and less than 1% also for a second time. No nuclear fusion was observed. © 1969 Springer-Verlag."

引用

页码：85 / &

共 7 条

[1] METHOD FOR THE ISOLATION OF PLANT PROTOPLASTS AND VACUOLES [J].