INTERACTIONS OF BERBERINE WITH POLY(A) AND TRANSFER-RNA

被引：62

作者：

NANDI, R ^{[1
]}

DEBNATH, D ^{[1
]}

MAITI, M ^{[1
]}

机构：

[1] INDIAN INST CHEM BIOL,CALCUTTA 700032,W BENGAL,INDIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1049卷 / 03期

关键词：

Alkaloid-nucleic acid interaction; Berberine; Poly(A); tRNA;

D O I：

10.1016/0167-4781(90)90107-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of berberine chloride with poly(A) and tRNA has been studied by various spectroscopic techniques. Binding parameters determined from spectrophotometric and spectrofluorimetric measurements by Scatchard analysis indicate a very high effective binding capacity of berberine to poly(A) as compared to DNA or tRNA. The circular dichroism studies show that binding of berberine to poly(A) causes a significant change in the circular dichroic spectrum of poly(A) itself, as manifested by (i) a decrease of both positive and negative bands and (ii) appearance of a conservative type of extrinsic circular dichroic spectrum in the wavelength range of 300-400 nm, while it does not cause any significant alteration to the A form structure of tRNA. It is concluded that berberine interacts stronger with poly (A) than DNA or tRNA. The results are interpreted in terms of its reported biological activities. © 1990.

引用

页码：339 / 342

页数：4

共 24 条

[1] SYNTHESIS OF SECRETED AND MEMBRANE-BOUND IMMUNOGLOBULIN-MU HEAVY-CHAINS IS DIRECTED BY MESSENGER-RNAS THAT DIFFER AT THEIR 3' ENDS [J].