MICROMETHOD FOR CHROMOSOME PREPARATIONS FROM CHINESE-HAMSTER LYMPHOCYTES

被引：4

作者：

DEJONG, B

ANDERS, GJPA

WIERDA, J

VANDERMEER, IH

ZIJLSTRA, J

机构：

来源：

CYTOGENETICS AND CELL GENETICS | 1979年 / 23卷 / 03期

关键词：

D O I：

10.1159/000131325

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Chromosome preparations from Chinese hamster lymphocytes were made by culturing for 2 to 6 days 50,000 to 800,000 lymphocytes in flat-bottomed Cooke microtiter plates in 0.1 or 0.2 ml Roswell Park Memorial Institute-1640 medium (RPMI-1640) supplemented with 5-40 μl PHA/ml, 20% fetal calf serum, and 40 μM 2-mercapto-ethanol. Depending on blood volume and cell concentrations used, it was possible to obtain up to 1400 well-spread metaphases from one venepuncture.

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页码：194 / 200

页数：7

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