PURIFICATION AND CHARACTERIZATION OF SOLUBLE HUMAN IL-6 RECEPTOR EXPRESSED IN CHO CELLS

被引：163

作者：

YASUKAWA, K

SAITO, T

FUKUNAGA, T

SEKIMORI, Y

KOISHIHARA, Y

FUKUI, H

OHSUGI, Y

MATSUDA, T

YAWATA, H

HIRANO, T

TAGA, T

KISHIMOTO, T

机构：

[1] OSAKA UNIV,INST MOLEC & CELLULAR BIOL,SUITA,OSAKA 565,JAPAN

[2] TOSOH CORP,BIOTECHNOL RES LAB,AYASE,KANAGAWA 252,JAPAN

[3] OSAKA UNIV,SCH MED,BIOMED RES CTR,KITA KU,OSAKA 530,JAPAN

[4] CHUGAI PHARMACEUT CO LTD,FUJI GOTEMBA RES LABS,GOTEMBA,SHIZUOKA 412,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 108卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123261

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An immunosorbent assay system to detect genetically engineered IL-6 receptor (IL-6R) was established, whereby soluble IL-6 receptor (sEL-6R) was detected in the culture medium when sIL-6R cDNA was transfected into COS1cells. A stably transformed Chinese hamster ovary (CHO) cell line constitutively expressing sIL-6R has been established. The recom-binant sIL-6R was purified to homogeneity by sequential filtration and chromatography of the culture medium. The recombinant aIL-6R augmented the sensitivity of Ml cells to IL-6 in growth inhibition assay in a dose-dependent manner. Furthermore, a radioisotope immunosorbent assay (RIA) utilizing recombinant sIL-6R was established which could detect IL-6 in a quantity as low as 10 ng/ml. © 1990 Copyright, 1990 by the Journal of Biochemistry.

引用

页码：673 / 676

页数：4

共 16 条

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