COMPLETE AMINO-ACID-SEQUENCE OF ENDO-BETA-N-ACETYLGLUCOSAMINIDASE FROM FLAVOBACTERIUM SP

被引：25

作者：

TAKEGAWA, K

MIKAMI, B

IWAHARA, S

MORITA, Y

YAMAMOTO, K

TOCHIKURA, T

机构：

[1] KYOTO UNIV,FOOD SCI RES INST,UJI,KYOTO 611,JAPAN

[2] KYOTO UNIV,FAC AGR,DEPT FOOD SCI & TECHNOL,KYOTO 606,JAPAN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 202卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb16359.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete amino acid sequence of endo-beta-N-acetylglucosaminidase from Flavobacterium sp. has been determined by analysis of peptides after cleavage with lysyl endopeptidase, pepsin and chymotrypsin. The protein consists of a single polypeptide chain consisting of 267 amino acid residues and a molecular mass of 27972 Da. The sequence of Flavobacterium endo-beta-N-acetylglucosaminidase is very close to that of the Streptomyces enzyme (endo-H), having 60% similarity and very similar hydropathy profiles. Similarities were also found between Flavobacterium endo-beta-N-acetylglucosaminidase and chitinases from Bacillus circulans, Serratia marcescens and Phaseolus vulgaris.

引用

页码：175 / 180

页数：6

共 14 条

[1] ETHYLENE-REGULATED GENE-EXPRESSION - MOLECULAR-CLONING OF THE GENES ENCODING AN ENDOCHITINASE FROM PHASEOLUS-VULGARIS [J].