ON-LINE FLUORESCENT DNA SEQUENCING WITH A SCANNING OPTICAL SYSTEM

被引：4

作者：

Fujii, Hidehiko ^{[1
]}

Nakamura, Shin ^{[1
]}

Hashimoto, Mitsuhiro ^{[1
]}

Kashiwagi, Katsuya ^{[1
]}

Watanabe, Toshihiro ^{[1
]}

机构：

[1] Shimadzu Corp, Biotechnol Instruments Dept, Nakagyo Ku, Kyoto 604, Japan

来源：

ANALYTICAL SCIENCES | 1991年 / 7卷

关键词：

laser induced fluorescence; DNA sequencing; gel electrophoresis;

D O I：

10.2116/analsci.7.Supple_649

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A laser induced fluorescence DNA sequencer is described. The excitation/objective optics are mounted on a mechanical scanner stage. The system allows the gel possible thinnest resulting in more than 450 mer peak resolution. Full two-dimensional data (gel width x time ladder pattern) are obtained, then can confirm up to gel entire raw data. All programs run on a multitasking operating system, which enables checking/processing obtained data whenever necessary even during electrophoresis. As a result, more than 500 bases sequencing was achieved from 0.5 mu g M13mp18 single stranded DNA template.

引用

页码：649 / 652

页数：4

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