CA2+-INDEPENDENT CYTOTOXICITY OF VIBRIO-PARAHAEMOLYTICUS THERMOSTABLE DIRECT HEMOLYSIN (TDH) ON INTESTINE-407, A CELL-LINE DERIVED FROM HUMAN EMBRYONIC INTESTINE

被引：31

作者：

TANG, GQ ^{[1
]}

IIDA, T ^{[1
]}

YAMAMOTO, K ^{[1
]}

HONDA, T ^{[1
]}

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,DEPT BACTERIAL INFECT,SUITA,OSAKA 565,JAPAN

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 134卷 / 2-3期

关键词：

CYTOTOXICITY; VIBRIO PARAHAEMOLYTICUS; THERMOSTABLE DIRECT HEMOLYSIN; INTESTINE; 407;

D O I：

10.1016/0378-1097(95)00411-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effects of Vibrio parahaemolyticus thermostable direct hemolysin on Intestine 407, a cell line derived from the intestine of human embryo, were investigated. The hemolysin was shown to be cytotoxic to Intestine 407. This cytotoxicity is accompanied by the damage of plasma membrane and lysosomes, as well as cellular degeneration in the form of large transparent blebs. Although an increase in cytosolic free Ca2+ due to the influx of extracellular Ca2+ was observed in cells treated with thermostable direct hemolysin, it was found to be irrelevant to any of the above effects. These results suggest that the effects of thermostable direct hemolysin observed in this study on Intestine 407 are not mediated by Ca2+-dependent pathways.

引用

页码：233 / 238

页数：6

共 12 条

[1]

ALBERTS B, 1989, MOL BIOL CELL, P459

[2]

ALBERTS B, 1989, MOL BIOL CELL, P71

[3]

[Anonymous], 1993, REV RES MED MICROBIO, DOI [10.1097/00013542-199304000-00006, DOI 10.1097/00013542-199304000-00006]

[4] REQUIREMENT OF CALCIUM-IONS FOR CELL DEGENERATION WITH A TOXIN (VIBRIOLYSIN) FROM VIBRIO-PARAHAEMOLYTICUS [J].

GOSHIMA, K ;

OWARIBE, K ;

YAMANAKA, H ;

YOSHINO, S .

INFECTION AND IMMUNITY, 1978, 22 (03) :821-832

[5] EXAMINATION OF THE TOXICITY OF SEVERAL PROTEIN TOXINS OF PLANT-ORIGIN USING BOVINE PULMONARY ENDOTHELIAL-CELLS [J].

GRIFFITHS, GD ;

LINDSAY, CD ;

UPSHALL, DG .

TOXICOLOGY, 1994, 90 (1-2) :11-27

[6] ASPECTS OF THE HEMOLYTIC REACTION INDUCED BY KANAGAWA HEMOLYSIN OF VIBRIO-PARAHAEMOLYTICUS [J].

HUNTLEY, JS ;

HALL, AC .

TOXICON, 1994, 32 (11) :1397-1412

[7]

ISHIYAMA M, 1993, CHEM PHARM BULL, V46, P1118

[8] NOVEL PATH TO APOPTOSIS - SMALL TRANSMEMBRANE PORES CREATED BY STAPHYLOCOCCAL ALPHA-TOXIN IN T-LYMPHOCYTES EVOKE INTERNUCLEOSOMAL DNA-DEGRADATION [J].

JONAS, D ;

WALEV, I ;

BERGER, T ;

LIEBETRAU, M ;

PALMER, M ;

BHAKDI, S .

INFECTION AND IMMUNITY, 1994, 62 (04) :1304-1312

[9] ACIDIFICATION OF THE ENDOCYTIC AND EXOCYTIC PATHWAYS [J].

MELLMAN, I ;

FUCHS, R ;

HELENIUS, A .

ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :663-700

[10] CALCIUM DEPENDENCE OF TOXIC CELL-DEATH - FINAL COMMON PATHWAY [J].

SCHANNE, FAX ;

KANE, AB ;

YOUNG, EE ;

FARBER, JL .

SCIENCE, 1979, 206 (4419) :700-702

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