HEAT-LABILE TOXIN OF BORDETELLA-PERTUSSIS PURIFIED BY PREPARATIVE ACRYLAMIDE GEL ELECTROPHORESIS

被引：25

作者：

NAKASE, Y

TAKATSU, K

TATEISHI, M

SEKIYA, K

KASUGA, T

机构：

[1] Department of Bacteriology, The Kitasato Institute, Tokyo

来源：

JAPANESE JOURNAL OF MICROBIOLOGY | 1969年 / 13卷 / 04期

关键词：

D O I：

10.1111/j.1348-0421.1969.tb00479.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The heat‐labile toxin (HLT) of Bordetella pertussis was purified by column chromatography on DEAE‐cellulose, salt fractionation and preparative acrylamide gel electrophoresis. The toxin obtained was confirmed to be free from hemagglutinins, protective antigens, histamin sensitizing factors, and K and O agglutinogens, and was shown to be homogenous by agar gel diffusion tests, ultracentrifugation, and electrophoresis. The minimal necrotic dose of the HLT in guinea pig was 0.01 μg dry weight. It was a protein in nature, contained a sugar moiety and possessed S value of 1.4. The toxicity of purified HLT was increased by mixing with a protein, such as rabbit serum. © jointly owned by Japanese society for Bacteriology and Society of Japanese Virologists

引用

页码：359 / &

共 22 条

[1] ANTIGENS OF BORDETELLA PERTUSSIS .2. PURIFICATION OF HEAT-LABILE TOXIN [J].