APPLICATION OF UNIVERSAL CALIBRATION IN GEL-PERMEATION CHROMATOGRAPHY FOR MOLECULAR-WEIGHT DETERMINATIONS OF PLANT-CELL WALL POLYMERS - COTTON FIBER

被引：87

作者：

TIMPA, JD

机构：

[1] Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, New Orleans, Louisiana 70179

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1991年 / 39卷 / 02期

关键词：

D O I：

10.1021/jf00002a010

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Methodology has been established to determine the molecular weight distribution of the cellulosic composition of cotton fibers without prior extraction or derivatization, process that can degrade polymers. Dimethylacetamide with lithium chloride is used for dissolution of samples. Commercially available, automated gel permeation chromatography (GPC) instrumentation with incorporation of a viscometer coupled with refractive index detection applies the universal calibration concept. With this configuration, GPC provides both a sensitive monitor of cotton fiber molecular weight distributions and valid molecular weight values. The weight-average molecular weight determined for cotton fiber was 11 000.

引用

页码：270 / 275

页数：6

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