THE 2.2-ANGSTROM CRYSTAL-STRUCTURE OF TRANSDUCIN-ALPHA COMPLEXED WITH GTP-GAMMA-S

被引：715

作者：

NOEL, JP

HAMM, HE

SIGLER, PB

机构：

[1] YALE UNIV, BOYER CTR MOLEC MED, DEPT MOLEC BIOPHYS & BIOCHEM, 295 CONGRESS AVE, NEW HAVEN, CT 06510 USA

[2] YALE UNIV, BOYER CTR MOLEC MED, HOWARD HUGHES MED INST, NEW HAVEN, CT 06510 USA

[3] UNIV ILLINOIS, DEPT PHYSIOL & BIOPHYS, CHICAGO, IL 60680 USA

[4] UNIV SASSARI, IST FISIOL GEN & CHIM BIOL, I-07100 SASSARI, ITALY

来源：

NATURE | 1993年 / 366卷 / 6456期

关键词：

D O I：

10.1038/366654a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The 2.2 angstrom crystal structure of activated rod transducin, G(talpha) . GTPgammaS, shows the bound GTPgammaS molecule occluded deep in a cleft between a domain structurally homologous to small GTPases and a helical domain unique to heterotrimeric G proteins. The structure, when combined with biochemical and genetic studies, suggests: how an activated receptor might open this cleft to allow nucleotide exchange; a mechanism for GTP-induced changes in effector and receptor binding surfaces; and a mechanism for GTPase activity not evident from previous data.

引用

页码：654 / 663

页数：10

共 52 条

[1] REGULATION OF DEACTIVATION OF PHOTORECEPTOR G-PROTEIN BY ITS TARGET ENZYME AND CGMP
ARSHAVSKY, VY
BOWNDS, MD
[J]. NATURE, 1992, 357 (6377) : 416 - 417
[2] ARTEMYEV NO, 1993, J BIOL CHEM, V268, P23611
[3] CRYSTAL-STRUCTURE OF ACTIVE ELONGATION-FACTOR TU REVEALS MAJOR DOMAIN REARRANGEMENTS
BERCHTOLD, H
RESHETNIKOVA, L
REISER, COA
SCHIRMER, NK
SPRINZL, M
HILGENFELD, R
[J]. NATURE, 1993, 365 (6442) : 126 - 132
[4] IDENTIFICATION OF EFFECTOR-ACTIVATING RESIDUES OF GS-ALPHA
BERLOT, CH
BOURNE, HR
[J]. CELL, 1992, 68 (05) : 911 - 922
[5] PHOSPHOLIPASE C-BETA-1 IS A GTPASE-ACTIVATING PROTEIN FOR GQ/11, ITS PHYSIOLOGICAL REGULATOR
BERSTEIN, G
BLANK, JL
JHON, DY
EXTON, JH
RHEE, SG
ROSS, EM
[J]. CELL, 1992, 70 (03) : 411 - 418
[6] BLUMER KJ, 1991, ANNU REV PHYSIOL, V53, P37
[7] THE GTPASE SUPERFAMILY - A CONSERVED SWITCH FOR DIVERSE CELL FUNCTIONS
BOURNE, HR
SANDERS, DA
MCCORMICK, F
[J]. NATURE, 1990, 348 (6297) : 125 - 132
[8] FREE R-VALUE - A NOVEL STATISTICAL QUANTITY FOR ASSESSING THE ACCURACY OF CRYSTAL-STRUCTURES
BRUNGER, AT
[J]. NATURE, 1992, 355 (6359) : 472 - 475
[9] BRUNGER AT, 1993, XPLOR VERSION 3 1 MA
[10] COMPLETE NUCLEOTIDE-SEQUENCES OF THE T24 HUMAN BLADDER-CARCINOMA ONCOGENE AND ITS NORMAL HOMOLOG
CAPON, DJ
CHEN, EY
LEVINSON, AD
SEEBURG, PH
GOEDDEL, DV
[J]. NATURE, 1983, 302 (5903) : 33 - 37

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