ELEMENTAL IMAGING OF CARTILAGE BY SCANNING-X-RAY MICROSCOPY

被引:9
作者
BUCKLEY, CJ
FOSTER, GF
BURGE, RE
ALI, SY
SCOTCHFORD, CA
KIRZ, J
RIVERS, ML
机构
[1] UNIV LONDON,ROYAL NATL ORTHOPAED HOSP,INST ORTHOPAED,STANMORE,MIDDX,ENGLAND
[2] SUNY STONY BROOK,DEPT PHYS,STONY BROOK,NY 11794
[3] UNIV CHICAGO,CTR ADV RADIAT SOURCES,CHICAGO,IL 60637
关键词
D O I
10.1063/1.1143804
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Elemental imaging via scanning transmission x-ray microscopy (STXM) and scanning fluorescence x-ray microscopy (SFXM) has been used to image calcium deposits in cartilage. In the case of STXM, 0.1-mu-m thick sections were imaged to investigate the proximity of calcium deposits in relation to chondrocyte cells. The resolution available was 0.5-mu-m, and field widths of up to 25-mu-m were used at this resolution. The resolution available in SFXM was 10-mu-m, and field widths of up to 2 mm were used at this resolution on 5-mu-m thick specimens. Together these techniques were used to map calcium deposits at the cellular level, and at the full tissue size level.
引用
收藏
页码:588 / 590
页数:3
相关论文
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