SULFATION OF VANILYLMANDELIC ACID AND HOMOVANILLIC-ACID BY LIVER, SMALL-INTESTINE AND BRAIN

被引：7

作者：

WONG, KP

机构：

[1] Department of Biochemistry, University of Singapore, Singapore, College Road

来源：

JOURNAL OF NEUROCHEMISTRY | 1979年 / 33卷 / 02期

关键词：

D O I：

10.1111/j.1471-4159.1979.tb05182.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— The sulpho‐conjugates of vanilylmandelic acid (VMA) and homovanillic acid (HVA) were biosynthesized in vitro from ATP and sodium sulphate and from 3′‐phosphoadenosine‐5′‐phosphosulphate (PAPS) obtained commercially or generated in vitro. Hydrolysis with sulphatase indicated the sulphate nature of these conjugates. In decreasing order of activity, the liver, small intestine and brain of various animals exhibited this sulpho‐conjugatory ability. The Km, values of VMA and HVA for the sulphotransferase of mouse liver were, respectively, 1740 and 93 μM. Competition studies suggested that the same enzyme may be involved in the sulphation of these two acids. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：517 / 520

页数：4

共 25 条

[1] CONJUGATION OF L-DOPA AND ITS METABOLITES AFTER ORAL AND INTRAVENOUS ADMINISTRATION TO PARKINSONIAN PATIENTS [J].