ASSAY OF ASPARTYLGLYCOSYLAMINASE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：20

作者：

KAARTINEN, V

MONONEN, I

机构：

[1] Department of Clinical Chemistry, Kuopio University Central Hospital

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 190卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90140-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An aspartylglycosylaminase assay based on high-performance liquid chromatographic analysis of the substrate aspartylglucosamine and product aspartate is described. Aspartylglucosamine and aspartate are derivatized with phenylisothiocyanate and resolved by reverse-phase chromatography. The detection limit for the compounds is 2 pmol. The method can be used for analysis of aspartylglycosylaminase activity in crude cell extracts and tissue samples. © 1990.

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页码：98 / 101

页数：4

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