COLORIMETRIC ASSAY OF LYSOGENIC INDUCTION DESIGNED FOR SCREENING POTENTIAL CARCINOGENIC AND CARCINOSTATIC AGENTS

被引：97

作者：

ELESPURU, RK ^{[1
]}

YARMOLINSKY, MB ^{[1
]}

机构：

[1] NCI,FREDERICK CANC RES CTR,CHEMOTHERAPY FERMENTAT LAB,FREDERICK,MD 21701

来源：

ENVIRONMENTAL MUTAGENESIS | 1979年 / 1卷 / 01期

关键词：

cancer chemotherapy; DNA damage; inductest; SOS response;

D O I：

10.1002/em.2860010113

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Simple, rapid colorimetric tests for lysogenic induction (the derepression of a latent bacterial virus) are described. A quantitative test and a more rapid semiquantitative test are based on the assay of the β‐galactosidase synthesized from a lacZ gene fused to an operon under lambda repressor control. These biochemical “inductests” are suitable for screening programs designed to detect agents that damage DNA and that are of potential interest in carcinogenesis and cancer chemotherapy. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company

引用

页码：65 / 78

页数：14

共 34 条

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