DEXTRAN SULFATE INHIBITS THE FUSION OF INFLUENZA-VIRUS WITH MODEL MEMBRANES, AND SUPPRESSES INFLUENZA-VIRUS REPLICATION INVIVO

被引：35

作者：

LUSCHERMATTLI, M ^{[1
]}

GLUCK, R ^{[1
]}

机构：

[1] SWISS SERUM & VACCINE INST BERNE,BERN,SWITZERLAND

来源：

ANTIVIRAL RESEARCH | 1990年 / 14卷 / 01期

关键词：

Dextran sulfate; Fluorescence spectroscopy; Influenza virus; Liposome; Membrane fusion;

D O I：

10.1016/0166-3542(90)90064-E

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of dextran sulfate and related compounds on the fusion of influenza A virus with model membranes, composed of dioleylphosphatidyl-choline and cholesterol (1:0.5), was investigated by a fusion assay based on de-quenching of fluorescence of octadecyl-rhodamine-HCl (R18). Dextran sulfate samples of molecular weight of 500 000, 8000 and 5000 were found to be potent inhibitors of the virusliposome fusion process. Polygalacturonic acid also showed anti-fusion activity, but to a lesser extent. Uncharged dextran, positively charged diethylaminoethyl-dextran, and the monomer glucosamin-1,6-disulfate were ineffective. It was shown that dextran sulfate interacts with the virus. Our results suggest that dextran sulfate binds to and inactivates the viral fusion protein. © 1990.

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页码：39 / 50

页数：12

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