DETECTION OF ESCHERICHIA-COLI AND SHIGELLA SPP IN WATER BY USING THE POLYMERASE CHAIN-REACTION AND GENE PROBES FOR UID

被引：269

作者：

BEJ, AK ^{[1
]}

DICESARE, JL ^{[1
]}

HAFF, L ^{[1
]}

ATLAS, RM ^{[1
]}

机构：

[1] PERKIN ELMER CORP,NORWALK,CT 06859

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 04期

关键词：

D O I：

10.1128/AEM.57.4.1013-1017.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method was developed for the detection of the fecal coliform bacterium Escherichia coli, using the polymerase chain reaction and gene probes, based on amplifying regions of the uid gene that code for beta-glucuronidase, expression of which forms the basis for fecal coliform detection by the commercially available Colilert method. Amplification and gene probe detection of four different regions of uid specifically detected E. coli and Shigella species, including beta-glucuronidase-negative strains of E. coli; no amplification was observed for other coliform and nonenteric bacteria.

引用

页码：1013 / 1017

页数：5

共 14 条

[1]

ATLAS RM, 1991, ASTM STP1102

[2]

Ausubel FM, 1987, CURRENT PROTOCOLS MO

[3] MULTIPLEX PCR AMPLIFICATION AND IMMOBILIZED CAPTURE PROBES FOR DETECTION OF BACTERIAL PATHOGENS AND INDICATORS IN WATER [J].