PRODUCTION OF ALKALINE XYLANASE BY A NEWLY ISOLATED ALKALIPHILIC BACILLUS SP STRAIN 41M-1

被引:51
作者
NAKAMURA, S
WAKABAYASHI, K
NAKAI, R
AONO, R
HORIKOSHI, K
机构
[1] Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo University of Technology, Yokohama, 227, 4259 Nagatsuta, Midori-ku
关键词
ALKALINE XYLANASE; ALKALIPHILIC BACILLUS;
D O I
10.1007/BF00327842
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Alkaliphilic Bacillus sp. strain 41M-1, isolated from soil, produced xylan-degrading enzymes extracellularly. Optimum pH for the crude xylanase preparation was about pH 9, confirming the production of novel alkaline xylanase(s) by the isolate. Xylanases were induced by xylan, but were not produced in the presence of xylose, arabinose or glucose. Xylanase productivity was influenced by culture pH, and production at pH 10.5 was higher than that at pH 8.0. Zymogram analaysis of the culture supernatant showed the alkaline xylanase with a molecular mass of 36 kDa.
引用
收藏
页码:221 / 224
页数:4
相关论文
共 11 条
  • [1] Claus D., Berkeley R.C.W., Genus Bacillus Cohn 1872, 174, Bergey's Manual of Systematic Bacteriology, pp. 1105-1139, (1986)
  • [2] Dey D., Hinge J., Shendye A., Rao M., Purification and properties of extracellular endoxylanases from alkaliphilic thermophilic Bacillus sp, Canadian Journal of Microbiology, 38, pp. 436-442, (1992)
  • [3] Honda H., Kudo T., Ikura Y., Horikoshi K., Two types of xylanases of alkalophilic Bacillus sp. No. C-125, Canadian Journal of Microbiology, 31, pp. 538-542, (1985)
  • [4] Horikoshi K., Atsukawa Y., Xylanase produced by alkalophilic Bacillus No. C-59-2, Agricultural and Biological Chemistry, 37, pp. 2097-2103, (1973)
  • [5] Laemmli U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, pp. 680-685, (1970)
  • [6] Morag E., Bayer E.A., Lamed R., Relationship of cellulosomal and noncellulosomal xylanases of Clostridium thermocellum to cellulose-degrading enzymes, Journal of Bacteriology, 172, pp. 6098-6105, (1990)
  • [7] Ohkoshi A., Kudo T., Mase T., Horikoshi K., Purification of three types of xylanases from an alkalophilic Aeromonas sp, Agricultural and Biological Chemistry, 49, pp. 3037-3038, (1985)
  • [8] Okazaki W., Akiba T., Horikoshi K., Akahoshi R., Production and properties of two types of xylanases from alkalophilic thermophilic Bacillus spp, Applied Microbiology and Biotechnology, 19, pp. 335-340, (1984)
  • [9] Sumner J.B., A more specific reagent for the determination of sugar in urine, Journal of Biological Chemistry, 47, pp. 393-395, (1925)
  • [10] Tsujibo H., Sakamoto T., Nishino N., Hasegawa T., Inamori Y., Purification and properties of three types of xylanases produced by an alkalophilic actinomycete, Journal of Applied Bacteriology, 69, pp. 398-405, (1990)