MICRONUCLEI FREQUENCY IN CIRCULATING ERYTHROCYTES FROM RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) SUBJECTED TO RADIATION, AN IMAGE-ANALYSIS AND FLOW CYTOMETRIC STUDY

被引：29

作者：

SCHULTZ, N ^{[1
]}

NORRGREN, L ^{[1
]}

GRAWE, J ^{[1
]}

JOHANNISSON, A ^{[1
]}

MEDHAGE, O ^{[1
]}

机构：

[1] SWEDISH UNIV AGR SCI, COLL VET MED, DEPT PATHOL, S-75007 UPPSALA, SWEDEN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 1993年 / 105卷 / 02期

关键词：

D O I：

10.1016/0742-8413(93)90196-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Rainbow trout (Oncorhynchus mykiss) were exposed to a single X-ray dose of 4 Gy. 2. The frequency of micronuclei in the peripheral erythrocytes was investigated at regular intervals up to 58 days after the exposure. 3. A flow cytometric method and a semi-automatic image analysis method were used to estimate the micronuclei frequency. 4. The results show that both methods can detect an increased frequency of micronuclei in peripheral erythrocytes from exposed fish. 5. However, the semi-automatic image analysis method was the most stable and sensitive.

引用

页码：207 / 211

页数：5

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