AGING, CARDIAC PROENKEPHALIN MESSENGER-RNA AND ENKEPHALIN PEPTIDES IN THE FISHER-344 RAT

被引:44
作者
CAFFREY, JL
BOLUYT, MO
YOUNES, A
BARRON, BA
ONEILL, L
CROW, MT
LAKATTA, EG
机构
[1] GERONTOL RES CTR, BALTIMORE, MD 21224 USA
[2] INST UNIV TECHNOL CLERMONT FERRAND, F-63172 CLERMONT FERRAND, FRANCE
关键词
CARDIAC OPIOID PEPTIDES; ENKEPHALINS; PROENKEPHALIN MESSENGER RNA; AGING;
D O I
10.1006/jmcc.1994.1085
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Cardiac proenkephalin (PENK) mRNA, methionine-enkephalin (ME) and leucine-enkephalin (LE) were determined from 2 days of age through senescence in Fisher 344 rats. Tissues were collected at 2 days, 2 weeks, 1, 2, 3, 7, 19, and either 22 or 27 months of age. Hearts were dissected, extracted and assaysed for ME and LE by radioimmunoassay (RIA) or for PENK mRNA by Northern blot analysis with a cDNA probe. Relative left ventricular (LV) PENK mRNA was low in 2 day animals and increased slowly between 2 weeks and 3 months of age. LV PENK mRNA then rose five to six-fold between 3 and 27 months of age. LV ME measurements were high in neonatal animals, declined to a nadir during development and then rose again as the animals matured and advanced in age. The pattern for right ventricular (RV) ME was similar. Atrial ME, also high at 2 days, declined thereafter and remained low. LE measurements in LV, RV and the atrial followed patterns similar to those described for ME. To evaluate for peptides contributed by cardiac nerves, 3, 7 and 22-month-old animals were acutely sympathectomized for 24 h with 6-hydroxydopamine. No decline in LV ME and LE was observed in the 6-hydroxydopamine treated animals. These data suggest several conclusions regarding mycardial enkephalinergic systems: (a) tissue enkephalin and PENK mRNA with advancing age, (b) tissue enkephalins may not strictly correlate with the relative abundance of PENK mRNA, and (c) most myocardial enkephalins are non-adrenergic in origin. The age-associated patterns in both PENK mRNA, ME and LE suggest that physiological, maturational or behavioral events between 3 and 7 months of age initiate the up-regulation and subsequent expansion of cardiac enkephalinergic systems. © 1994 Academic Press Limited.
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收藏
页码:701 / 711
页数:11
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