EXPRESSION OF IL-2 AND IL-4 IN T-LYMPHOCYTES TRANSFORMED BY HERPESVIRUS SAIMIRI

被引：18

作者：

CHOU, CS

MEDVECZKY, MM

GECK, P

VERCELLI, D

MEDVECZKY, PG

机构：

[1] UNIV S FLORIDA,COLL MED,DEPT MED MICROBIOL & IMMUNOL,TAMPA,FL 33612

[2] CHILDRENS HOSP,DIV ALLERGY & IMMUNOL,BOSTON,MA 02115

[3] HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115

来源：

VIROLOGY | 1995年 / 208卷 / 02期

关键词：

D O I：

10.1006/viro.1995.1172

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Herpesvirus saimiri (H. saimiri) is a highly oncogenic lymphotropic herpesvirus which can immortalize T lymphocytes and cause tumors in rabbits and New World monkeys. T cells infected with strain 484-77 of group C express four viral U-like small RNAs (HSUR1-4) and a 1.2-kb mRNA which encodes open reading frames ORF-1 and ORF-2. ORF-I encodes a collagen-like oncoprotein. Deletion mutation analysis showed that ORF-1 and ORF-2 are essential for IL-2 independent growth of human T cells infected with H. saimiri. An earlier study also demonstrated that H. saimiri-immortalized cells carry functional IL-2 receptors. The work presented in this report investigated whether IL-2 and IL-4 is produced by H. saimiri-immortalized T lymphocytes. Both IL-2 mRNA and IL-4 mRNA were detected in various monkey T cells as well as human peripheral blood lymphocytes infected with wild-type H. saimiri. Secretion of IL-2 was suggested by cyclosporin A inhibition. IL-4 secretion by monkey T cell cultures was demonstrated by a bioassay and inhibition of bioactivity by an antibody to IL-4. The data also show that recombinant IL-4 stimulate H. saimiri-immortalized T cells; thus, IL-4 receptors are expressed. However, antibodies to human IL-4, IL-4 receptor, or soluble IL-4 receptor did not curtail growth of transformed cells. T cells infected with ORF-1 and ORF-2 deletion mutants expressed no detectable IL-2 mRNA. ORF-1, ORF-2, HSUR1, and HSUR2, were all essential for expression of IL-4 mRNA. These data are consistent with the hypothesis that H. saimiri-immortalized monkey and human T lymphocytes proliferate through autocrine secretion of IL-2 and that ORF-1, ORF-2, and HSUR sequences of the virus are involved in expression of lymphokines. (C) 1995 Academic Press, Inc.

引用

页码：418 / 426

页数：9

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