SIMULTANEOUS DETERMINATION OF MIDAZOLAM AND FLUMAZENIL CONCENTRATIONS IN HUMAN PLASMA BY GAS-CHROMATOGRAPHY

被引：24

作者：

FISHER, LE

PERCH, S

BONFIGLIO, MF

GEERS, SM

机构：

[1] SUMMA HLTH SYST,DEPT LAB MED & PATHOL,AKRON,OH 44309

[2] OHIO NO UNIV,DEPT PHARM PRACTICE,ADA,OH 45810

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 665卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00510-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gas chromatographic method for the simultaneous determination of midazolam and flumazenil concentrations in human plasma has been developed, using clinazolam as the internal standard. A mixture of chloroform and ethyl acetate (80:20) was used to extract the two compounds. A 2-mu l volume of the reconstituted sample was injected using a 1:20 split injection mode. Intra-day coefficients of variation ranged from 2 to 6.9%. The assay was linear over the range 3-1000 ng/ml. This assay was subsequently used to analyze samples from a human pharmacokinetic study.

引用

页码：217 / 221

页数：5

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