SMALL-VOLUME THIN-LAYER SPECTROELECTROCHEMICAL CELL FOR THE STUDY OF BIOLOGICAL COMPONENTS

被引：50

作者：

ANDERSON, CW ^{[1
]}

HALSALL, HB ^{[1
]}

HEINEMAN, WR ^{[1
]}

机构：

[1] UNIV CINCINNATI,DEPT CHEM,CINCINNATI,OH 45221

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 93卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0003-2697(79)80164-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A small-volume (∼60 μl) spectroelectrochemical cell was constructed and characterized.The working electrode is An minigrid, and the spectral window is from 170 nm to 2 μm. The solution is held in a thin layer around the working electrode, providing the advantages of thin-layer electrochemistry. The cell can be constructed from common materials in about 6-h working time. The cell also has flow-through and vacuum capabilities, with only minor modifications. Characterization of the cell was done by cyclic voltammetry and coulometry on ferri-ferrocyanide and spectroelectrochemistry on cytochrome c and o-tolidine. The optical and electrochemical windows of this cell, as well as its convenient and versatile operation, make it useful for the study of a wide variety of biological redox components. © 1979 Academic Press, Inc.

引用

页码：366 / 372

页数：7

共 19 条

[1] TEMPERATURE-DEPENDENCE OF REDOX POTENTIAL OF HORSE HEART CYTOCHROME-C IN SODIUM-CHLORIDE SOLUTIONS [J].

ANDERSON, CW ;

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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 76 (02) :339-344

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HAWKRIDGE, FM ;

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[5] MEASUREMENT OF ENZYME-EDEGREES' VALUES BY OPTICALLY TRANSPARENT THIN-LAYER ELECTROCHEMICAL CELLS [J].

HEINEMAN, WR ;

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GOELZ, JF .

ANALYTICAL CHEMISTRY, 1975, 47 (01) :79-84

[6] COMBINATION OF OPTICAL AND ELECTROCHEMICAL TECHNIQUES FOR STUDIES OF REDOX CHEMISTRY [J].

HEINEMAN, WR .

ANALYTICAL CHEMISTRY, 1978, 50 (03) :A390-&

[7] THIN-LAYER SPECTROELECTROCHEMICAL STUDY OF VITAMIN B-12 AND RELATED COBALAMIN COMPOUNDS IN AQUEOUS-MEDIA [J].

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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1976, 98 (09) :2469-2477

[8] SPECULAR REFLECTANCE IN THIN-LAYER ELECTROCHEMISTRY [J].

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ANALYTICAL CHEMISTRY, 1970, 42 (01) :12-+

[9] The oxidation potential of the system potassium ferrocyanide-potassium ferricyanide at various ionic strengths [J].

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JOURNAL OF PHYSICAL CHEMISTRY, 1935, 39 (07) :945-954

[10] EFFECT OF BULK SOLVENT STRUCTURE ON TEMPERATURE-DEPENDENCE OF REDUCTION POTENTIAL OF CYTOCHROME-C [J].

KREISHMAN, GP ;

ANDERSON, CW ;

SU, CH ;

HALSALL, HB ;

HEINEMAN, WR .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1978, 5 (01) :196-203

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