USE OF A CELLULASE-ENCODING GENE PROBE TO REVEAL RESTRICTION FRAGMENT LENGTH POLYMORPHISMS AMONG RUMINAL STRAINS OF BACTEROIDES-SUCCINOGENES

被引：16

作者：

FLINT, HJ ^{[1
]}

MCPHERSON, CA ^{[1
]}

AVGUSTIN, G ^{[1
]}

STEWART, CS ^{[1
]}

机构：

[1] EDVARD KARDELJ UNIV, FAC BIOTECH, DEPT ZOOTECH, YU-61230 DOMZALE, YUGOSLAVIA

来源：

CURRENT MICROBIOLOGY | 1990年 / 20卷 / 01期

关键词：

D O I：

10.1007/BF02094027

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A cloned DNA fragment specifying an endoglucanase from Bacteroides succinogenes strain BL2 was shown to hybridize under nonstringent conditions to different BamH1 fragments of chromosomal DNA from each of five rumen strains of B. succinogenes. Direct binding of BL2 total chromosomal DNA to DNA from the other four strains was between 16% and 42% of homologous binding, confirming a high degree of interstrain divergence. Bacteroides succinogenes BL2 chromosomal DNA did not show detectable hybridization with DNA from any of 15 strains of 11 other species of rumen bacteria, in tests carried out with NaOH-lysed cells on filters. © 1990 Springer-Verlag New York Inc.

引用

页码：63 / 67

页数：5

共 16 条

[1] USE OF A UNIQUE GENE SEQUENCE AS A PROBE TO ENUMERATE A STRAIN OF BACTEROIDES-RUMINICOLA INTRODUCED INTO THE RUMEN [J].

ATTWOOD, GT ;

LOCKINGTON, RA ;

XUE, GP ;

BROOKER, JD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (02) :534-539

[2] POLYACRYLAMIDE-GEL ELECTROPHORESIS OF BACTEROIDES-SUCCINOGENES [J].

BEGBIE, R ;

STEWART, CS .

CANADIAN JOURNAL OF MICROBIOLOGY, 1984, 30 (06) :863-866

[3]

Crosby B, 1984, 5TH P CAN BIOEN R D, P573

[4] CLONING OF THE ARG-12 GENE OF NEUROSPORA-CRASSA AND REGULATION OF ITS TRANSCRIPT VIA CROSS-PATHWAY AMINO-ACID CONTROL [J].

FLINT, HJ ;

WILKENING, J .

MOLECULAR & GENERAL GENETICS, 1986, 203 (01) :110-116

[5] PLASMID-ASSOCIATED TRANSFER OF TETRACYCLINE RESISTANCE IN BACTEROIDES-RUMINICOLA [J].

FLINT, HJ ;

THOMSON, AM ;

BISSET, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (04) :855-860

[6]

Johnson JL, 1981, MANUAL METHODS GENER, P450

[7]

Maniatis T., 1982, MOL CLONING LAB MANU

[8] DEOXYRIBONUCLEIC-ACID RELATEDNESS AMONG STRAINS OF THE SPECIES BUTYRIVIBRIO-FIBRISOLVENS [J].

MANNARELLI, BM .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1988, 38 (04) :340-347

[9] TRANSFER OF BACTEROIDES-SUCCINOGENES (HUNGATE) TO FIBROBACTER GEN-NOV AS FIBROBACTER-SUCCINOGENES COMB NOV AND DESCRIPTION OF FIBROBACTER INTESTINALIS SP-NOV [J].

MONTGOMERY, L ;

FLESHER, B ;

STAHL, D .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1988, 38 (04) :430-435

[10] RAPID AND CORRECT IDENTIFICATION OF INTESTINAL BACTEROIDES SPP WITH CHROMOSOMAL DNA PROBES BY WHOLE-CELL DOT BLOT HYBRIDIZATION [J].

MOROTOMI, M ;

OHNO, T ;

MUTAI, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (05) :1158-1162

← 1 2 →