COMPARISON OF CYTOCHROME-P-450 LEVELS IN ADULT-RAT LIVER, POSTNATAL RAT-LIVER, AND PRIMARY CULTURES OF POSTNATAL RAT HEPATOCYTES

被引：24

作者：

ACOSTA, D ^{[1
]}

ANUFORO, DC ^{[1
]}

MCMILLIN, R ^{[1
]}

SOINE, WH ^{[1
]}

SMITH, RV ^{[1
]}

机构：

[1] UNIV TEXAS,INST DRUG DYNAM,COLL PHARM,AUSTIN,TX 78712

来源：

LIFE SCIENCES | 1979年 / 25卷 / 16期

关键词：

D O I：

10.1016/0024-3205(79)90419-3

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A system of primary cultures of postnatal rat hepatocytes has been developed to serve as an experimental model for drug metabolism and toxicity investigations. The purpose of this study was to examine the reported loss of cytochrome P-450 of hepatocytes when placed in culture and to compare activity in culture to intact liver and freshly isolated hepatocytes. A medium enriched with several hormones and a system of floating filters as a substratum for cell attachment were investigated as methods to reduce the expected loss of cytochrome P-450. When compared to initial values of cytochrome P-450 in whole liver and isolated hepatocytes, these methods failed to prevent the reduction of cytochrome P-450 in culture. However, our results compare favorably with other values reported in the literature. © 1979.

引用

页码：1413 / 1418

页数：6

共 32 条

[1]

ACOSTA D, 1978, IN VITRO CELL DEV B, V14, P428

[2]

ACOSTA D, 1979, FED PROC, V38, P366

[3]

ANUFORO DC, 1978, IN VITRO CELL DEV B, V14, P981

[4] STUDIES ON PERSISTENCE OF DIFFERENTIATED FUNCTIONS IN RAT HEPATOCYTES SET INTO PRIMARY TISSUE-CULTURES - SPECIFIC BINDING OF BILIRUBIN-H-3 AFTER 8 DAYS OF STAYING INVITRO [J].