CHARACTERIZATION OF 3 MONOCLONAL-ANTIBODIES THAT RECOGNIZE THE INTERFERON-ALPHA-2 RECEPTOR

被引：65

作者：

COLAMONICI, OR ^{[1
]}

DALESSANDRO, F ^{[1
]}

DIAZ, MO ^{[1
]}

GREGORY, SA ^{[1
]}

NECKERS, LM ^{[1
]}

NORDAN, R ^{[1
]}

机构：

[1] NCI,MED BRANCH,MOLEC & CELL BIOL SECT,BETHESDA,MD 20892

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 18期

关键词：

D O I：

10.1073/pnas.87.18.7230

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The interferon system plays an important role in the control of viral infections and cell proliferation. These effects are mediated through the interaction of interferons with specific cell surface receptors. We report here the development of monoclonal antibodies against one of the subunits of the interferon α receptor. These antibodies detect a 110-kDa protein in surface-labeled cells and in Western blots, and 130- and 210-kDa bands after crosslinking to iodinated interferon α2. No other subunits are disulfide-linked to the 130-kDa subunit or are coprecipitated by these antibodies. Analysis by two-dimensional gel electrophoresis revealed that the pI of this subunit is 3.5-5.0. We suggest that the protein recognized by these monoclonal antibodies be named the α subunit of the interferon α receptor.

引用

页码：7230 / 7234

页数：5

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