EXPRESSION OF THE CELLULASE (FI-CMCASE) GENE OF ASPERGILLUS-ACULEATUS IN ESCHERICHIA-COLI

被引：11

作者：

OOI, T

MINAMIGUCHI, K

KAWAGUCHI, T

OKADA, H

MURAO, S

ARAI, M

机构：

[1] UNIV OSAKA PREFECTURE, COLL AGR, DEPT AGR CHEM, SAKAI, OSAKA 593, JAPAN

[2] KUMAMOTO INST TECHNOL, DEPT APPL MICROBIAL TECHNOL, IKEDA, KUMAMOTO 860, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 11期

关键词：

D O I：

10.1271/bbb.57.1960

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

FI-CMCase cDNA of Aspergillus aculeatus was expressed in Escherichia coli by using the tac promoter of E. coli. Transformants of E. coli harboring a plasmid pHEM06 containing mature form FI-CMCase cDNA produced FI-CMCase in the cytoplasm of the cells. The enzyme from E. coli cells was purified to yield 56% and it was immunological identical to that of FI-CMCase purified from A. aculeatus.

引用

页码：1960 / 1961

页数：2

共 7 条

[1]

BULLOCK WO, 1987, BIOTECHNIQUES, V5, P376

[2] CONSTRUCTION OF A PLASMID VECTOR FOR THE REGULATABLE HIGH-LEVEL EXPRESSION OF EUKARYOTIC GENES IN ESCHERICHIA-COLI - AN APPLICATION TO OVERPRODUCTION OF CHICKEN LYSOZYME [J].