ELECTROPERMEABILIZED PLATELETS - A PREPARATION TO STUDY EXOCYTOSIS

被引:6
作者
KNIGHT, DE [1 ]
SCRUTTON, MC [1 ]
机构
[1] UNIV LONDON KINGS COLL,DIV LIFE SCI,LONDON WC2R 2LS,ENGLAND
基金
美国国家科学基金会;
关键词
D O I
10.1016/0076-6879(93)21012-W
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This chapter describes the procedures necessary for successful preparation of platelets rendered permeable by a high-voltage electric field, the potential and limitations of this experimental system, and some of the results that have been obtained from experiments in which it has been used. Platelet activation, caused by many agonists, includes the secretion, by exocytosis, of various factors from this cell. Because platelet secretion is triggered by a range of secretagogues it has been suggested that multiple, and possibly independent, pathways may be involved in the exocytotic mechanism. Electroporation of the platelet plasma membrane has provided new insight into this area. One preparation that has generated considerable interest because of the central role of the cell in many pathological processes involves the permeabilized blood platelet. The studies performed thus far with this method suggest that selective permeabilization of the plasma membrane can be achieved with minimal damage to other parts of the platelets. © 1993, Elsevier Inc. All rights reserved.
引用
收藏
页码:123 / 138
页数:16
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