MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF RESTRICTION ENZYME-DIGESTED PLASMID DNA USING AN ACTIVE NAFION SUBSTRATE

被引：40

作者：

BAI, J

LIU, YH

LUBMAN, DM

SIEMIENIAK, D

机构：

[1] UNIV MICHIGAN, DEPT CHEM, ANN ARBOR, MI 48109 USA

[2] UNIV MICHIGAN, MED CTR, HOWARD HUGHES MED INST, ANN ARBOR, MI 48109 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 09期

关键词：

D O I：

10.1002/rcm.1290080904

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization using a 3-hydroxypicolinic acid matrix from an active Nafion substrate has been used for detection of restriction enzyme-digested double-stranded plasmid DNA using time-of-flight mass spectrometry. DNA strands of up to 267 base pairs were detected with minimal sample purification, although only as species corresponding to single-stranded DNA.

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页码：687 / 691

页数：5

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