PRODUCTION OF PECTIN LYASE BY PENICILLIUM-GRISEOROSEUM CULTURED ON SUCROSE AND YEAST EXTRACT FOR DEGUMMING OF NATURAL FIBERS

被引:23
作者
BARACATPEREIRA, MC [1 ]
COELHO, JLC [1 ]
SILVA, DO [1 ]
机构
[1] UNIV FED VICOSA,DEPT MICROBIOL,BR-36570000 VICOSA,MG,BRAZIL
关键词
D O I
10.1111/j.1472-765X.1994.tb00824.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Sucrose, a non-pectic carbon source, with yeast extract (YE) added was able to support the production of pectin lyase (PL) by Penicillium griseoroseum Dierckx. However, in the absence of YE, the fungus did not produce PL but grew and caused a marked reduction in culture medium pH. Furthermore, in the absence of YE, only a culture medium with a high buffering capacity permitted the production of PL in the presence of pectin. On the other hand, in the presence of 0.06% YE and of 0.1% pectin, the fungus produced maximum growth and specific PL activity during a 48-h period of culture, with a small variation in medium pH. In the absence of sucrose, YE concentrations from 0 to 0.16% did not support enzyme production, indicating synergism between sucrose and YE for production of the enzyme.
引用
收藏
页码:127 / 129
页数:3
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