SYNERGISTIC INHIBITION BY BQ-123 AND BQ-788 OF ENDOTHELIN-1-INDUCED CONTRACTIONS OF THE RABBIT PULMONARY-ARTERY

In the rabbit isolated pulmonary artery, neither the ET(A) receptor antagonist, BQ-123 (10 mu M), nor the ET(B) receptor antagonist, BQ-788 (10 mu M), inhibited the contractions induced by 1 nM endothelin-1 (ET-1). However, the combination of BQ-123 and BQ-788 completely inhibited the ET-1-induced contraction. In contrast, the ET(B)-selective agonist, sarafotoxin S6c (1 nM)-induced contraction was completely inhibited by BQ-788 but not by BQ-123. In receptor binding assays, [I-125]-ET-1 specific binding to pulmonary arterial membranes was inhibited by BQ-123 (1 mu M) by approximately 20% and additive treatment with BQ-788 (1 mu M) completely inhibited the BQ-123-resistant component of[I-125]-ET-1 specific binding. The present study demonstrates synergistic inhibition by BQ-123 and BQ-788 of ET-1-induced contraction of the rabbit pulmonary artery and the coexistence of ET(A) and ET(B) receptors, suggesting that the activation of either only ET(A) or only ET(B) receptors may be sufficient to cause complete vasoconstriction. Therefore, blockade of both receptor subtypes would be necessary for the inhibition of some ET(A)/ET(B) composite types of responses.