POLYPYRIMIDINE POLYPURINE SEQUENCE IN PLASMID DNA ENHANCES FORMATION OF DIMER MOLECULES IN ESCHERICHIA-COLI - DIMERIZATION OF PLASMID DNA IN ESCHERICHIA-COLI

被引：6

作者：

KATO, M

机构：

[1] Department of Life Science, College of Integrated Arts and Sciences, University of Osaka Prefecture, Sakai, 593

来源：

MOLECULAR BIOLOGY REPORTS | 1993年 / 18卷 / 03期

关键词：

DNA TRIPLE HELIX; ESCHERICHIA-COLI; PLASMID DNA; POLYPYRIMIDINE POLYPURINE BIAS; RECOMBINATION; REPLICATION;

D O I：

10.1007/BF01674429

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Formation of dimer molecules of a recombinant plasmid, pTIR10, which carries a pyrimidine/purine-biased stretch occurs about 6-fold more efficiently than for the control plasmid pUC19 in Escherichia coli strain JM107. Since pyrimidine/purine-biased sequences have a potential to form unusual DNA structures, this observation suggests that the inserted sequence affects the replication process of plasmid DNA, probably by forming a triple helix under physiological conditions.

引用

页码：183 / 187

页数：5

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