TRANSIENT EXPRESSION OF BETA-GLUCURONIDASE FOLLOWING BIOLISTIC DELIVERY OF FOREIGN DNA INTO COFFEE TISSUES

被引：29

作者：

VANBOXTEL, J

BERTHOULY, M

CARASCO, C

DUFOUR, M

ESKES, A

机构：

[1] CATIE,7170 TURRIABA,COSTA RICA

[2] CIRAD,CP,F-34042 MONTPELLIER,FRANCE

来源：

PLANT CELL REPORTS | 1995年 / 14卷 / 12期

关键词：

D O I：

10.1007/BF00232915

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Some conditions related to the transient expression of beta-glucuronidase in biolistically-treated Coffea spp. tissues were investigated, and subsequently used in a promoter study. Bombardments were performed on different types of tissue (leaves, somatic embryos and suspension cultures) of genotypes of C. arabica, C. canephora and Arabusta, using 4 different promoter sequences. Tobacco leaves were used as a comparison. In general, similar large variation and mean values of transient expression were observed between coffee and tobacco leaves. With regard to the coffee tissues effect, transient expression was best detectable and most frequently observed with bombarded leaves of microcuttings. Disturbing endogenous light blue staining was found with control treatments of somatic embryos. For the three coffee species tested, the most effective promoter was the EF1 alpha-A1 promoter of Arabidopsis thaliana.

引用

页码：748 / 752

页数：5

共 30 条

[1] PLANT-REGENERATION FROM PROTOPLASTS OF EMBRYOGENIC CELL-SUSPENSIONS OF COFFEA-ARABICA L CV CATURRA [J].