DNA MINOR-GROOVE BINDING OF CROSS-LINKED LEXITROPSINS - EXPERIMENTAL CONDITIONS REQUIRED TO OBSERVE THE COVALENTLY-LINKED WPPW (GROOVE WALL-PEPTIDE-PEPTIDE-GROOVE WALL) MOTIF

被引：17

作者：

CHEN, YH ^{[1
]}

LOWN, JW ^{[1
]}

机构：

[1] UNIV ALBERTA, DEPT CHEM, EDMONTON, AB T6G 2G2, CANADA

来源：

BIOPHYSICAL JOURNAL | 1995年 / 68卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/S0006-3495(95)80382-6

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A theoretical analysis of binding interactions between covalently cross-linked lexitropsins and DNA is undertaken, in which a novel cyclic symmetric 2:2 dimeric lexitropsin-DNA-binding model is proposed. Applicability of commonly used techniques including NMR, quantitative footprinting, CD, and ethidium fluorometry to differentiate the covalently linked WPPW (groove Wall-Peptide-Peptide-groove Wall) from a 2:2 cross-linked lexitropsin-DNA duplex structure is examined.

引用

页码：2041 / 2048

页数：8

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