Functional analysis of human spuma retrovirus genome

被引：8

作者：

Adachi, A ^{[1
]}

Sakai, H ^{[1
]}

Tokunaga, K ^{[1
]}

Kawamura, M ^{[1
]}

机构：

[1] KYOTO UNIV,INST VIRUS RES,DEPT VIRAL ONCOL,LAB GENE ANAL,SAKYO KU,KYOTO 606,JAPAN

来源：

VIRUS GENES | 1995年 / 11卷 / 01期

关键词：

HSRV; mutant; Eel; transcription; Rev; Rex;

D O I：

10.1007/BF01701657

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Human spuma retrovirus (HSRV) belongs to retroviruses that possess a complex genome organization. HSRV carries at least three extra genes in the region between env and the 3' long terminal repeat, which are not found in simple retroviruses. Via alternative splicing, these HSRV genes can encode several proteins. To genetically study the requirements of these viral proteins for viral replication in tissue cultures, a number of mutant viruses were constructed from an infectious molecular clone of HSRV. All mutants grew normally in the cell lines tested, except for those lacking transcriptional transactivator activity. By reporter-based transient assay systems, no Rev/Rex equivalent was detected in the HSRV proteins.

引用

页码：15 / 20

页数：6

共 18 条

[1] PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE [J].

ADACHI, A ;

GENDELMAN, HE ;

KOENIG, S ;

FOLKS, T ;

WILLEY, R ;

RABSON, A ;

MARTIN, MA .

JOURNAL OF VIROLOGY, 1986, 59 (02) :284-291

[2] FUNCTIONAL-ANALYSIS OF HUMAN FOAMY VIRUS ACCESSORY READING FRAMES [J].

BAUNACH, G ;

MAURER, B ;

HAHN, H ;

KRANZ, M ;

RETHWILM, A .

JOURNAL OF VIROLOGY, 1993, 67 (09) :5411-5418

[3] MECHANISM OF ACTION OF REGULATORY PROTEINS ENCODED BY COMPLEX RETROVIRUSES [J].

CULLEN, BR .

MICROBIOLOGICAL REVIEWS, 1992, 56 (03) :375-394

[4]

FLUGEL RM, 1991, J ACQ IMMUN DEF SYND, V4, P739

[5] HUMAN FOAMY VIRUS POLYPEPTIDES - IDENTIFICATION OF ENV AND BEL GENE-PRODUCTS [J].

GIRON, ML ;

ROZAIN, F ;

DEBONSGUILLEMIN, MC ;

CANIVET, M ;

PERIES, J ;

EMANOILRAVIER, R .

JOURNAL OF VIROLOGY, 1993, 67 (06) :3596-3600

[6] RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS [J].

GORMAN, CM ;

MOFFAT, LF ;

HOWARD, BH .

MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (09) :1044-1051

[7] CHARACTERIZATION OF THE TRANSCRIPTIONAL TRANS ACTIVATOR OF HUMAN FOAMY RETROVIRUS [J].

KELLER, A ;

PARTIN, KM ;

LOCHELT, M ;

BANNERT, H ;

FLUGEL, RM ;

CULLEN, BR .

JOURNAL OF VIROLOGY, 1991, 65 (05) :2589-2594

[8] SEQUENCE-ANALYSIS OF THE SIMIAN FOAMY VIRUS TYPE-1 GENOME [J].

KUPIEC, JJ ;

KAY, A ;

HAYAT, M ;

RAVIER, R ;

PERIES, J ;

GALIBERT, F .

GENE, 1991, 101 (02) :185-194

[9] CONSTRUCTION OF AN INFECTIOUS DNA CLONE OF THE FULL-LENGTH HUMAN SPUMARETROVIRUS GENOME AND MUTAGENESIS OF THE BEL-1 GENE [J].

LOCHELT, M ;

ZENTGRAF, H ;

FLUGEL, RM .

VIROLOGY, 1991, 184 (01) :43-54

[10] ANALYSIS OF SPLICING PATTERNS OF HUMAN SPUMARETROVIRUS BY POLYMERASE CHAIN-REACTION REVEALS COMPLEX RNA STRUCTURES [J].

MURANYI, W ;

FLUGEL, RM .

JOURNAL OF VIROLOGY, 1991, 65 (02) :727-735

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