CALF SPLEEN PURINE NUCLEOSIDE PHOSPHORYLASE - PURIFICATION, SEQUENCE AND CRYSTAL-STRUCTURE OF ITS COMPLEX WITH AN N(7)-ACYCLOGRANOSINE INHIBITOR

被引：57

作者：

BZOWSKA, A

LUIC, M

SCHRODER, W

SHUGAR, D

SAENGER, W

KOELLNER, G

机构：

[1] FREE UNIV BERLIN,INST KRISTALLOG,D-14195 BERLIN,GERMANY

[2] UNIV WARSAW,INST EXPTL PHYS,DEPT BIOPHYS,PL-02089 WARSAW,POLAND

[3] RUDJER BOSKOVIC INST,ZAGREB 41000,CROATIA

[4] FREE UNIV BERLIN,INST BIOCHEM,D-14195 BERLIN,GERMANY

来源：

FEBS LETTERS | 1995年 / 367卷 / 03期

关键词：

PURINE NUCLEOSIDE PHOSPHORYLASE; AMINO ACID SEQUENCE; CRYSTALLIZATION; X-RAY CRYSTALLOGRAPHY; MOLECULAR REPLACEMENT; N(7)-BETA-D-PURINE NUCLEOSIDE;

D O I：

10.1016/0014-5793(95)00540-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calf spleen purine nucleoside phosphorylase was purified to homogeneity and its amino acid sequence was determined, The complex of the enzyme with an N(7)-acycloguanosine inhibitor crystallized in the cubic space group P2(1)3, with unit cell dimension a = 94.02 Angstrom and one monomer in the asymmetric crystal unit, The biologically active trimer is formed by the crystallographic three-fold axis, The structure was solved by molecular replacement methods, using the model of the human erythrocyte enzyme, and refined at a resolution of 2.9 Angstrom to an R-factor of 0.21, The orientation of the inhibitor at the active site is examined in relation to the catalytic activity of the enzyme in the phosphorolysis of N(7)-beta-D-purine nucleosides.

引用

页码：214 / 218

页数：5

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