MICRO METHOD FOR DETECTION OF FORMYL AND ACETYL GROUPS IN PROTEINS

被引：94

作者：

SCHMER, G

KREIL, G

机构：

[1] Institute for Molecular Biology, Austrian Academy of Sciences, A-1090 Vienna

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 29卷 / 02期

关键词：

D O I：

10.1016/0003-2697(69)90301-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A micro method for the detection of N-formyl and N-acetyl groups in polypeptides is described. This method is based on the following principle: the acylhydrazine obtained after cleavage with anhydrous hydrazine is incubated with dansyl chloride. The reaction product, a 1-acyl-2-dansyl-hydrazine, can be identified by two-dimensional thin-layer chromatography. This method has been tested with a number of peptides and proteins known to contain formyl or acetyl groups at the amino end. © 1969.

引用

页码：186 / &

共 16 条

[1] N-FORMYLMETHIONYL-SRNA AS INITIATOR OF PROTEIN SYNTHESIS [J].