THE COMPLETE SEQUENCE OF THE 8.2KB SEGMENT LEFT OF MAT ON CHROMOSOME-III REVEALS 5 ORFS, INCLUDING A GENE FOR A YEAST RIBOKINASE

被引：92

作者：

THIERRY, A

FAIRHEAD, C

DUJON, B

机构：

[1] Unité de Génétique Moléculaire Des Levures (Ura 149 du Cnrs), Institut Pasteur, Paris, F-75724

来源：

YEAST | 1990年 / 6卷 / 06期

关键词：

ARS; Chromosome III; gene disruption; ribokinase; sequencing;

D O I：

10.1002/yea.320060609

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the DNA sequence of a segment of chromosome III extending over 8·2 kb. The sequence was determined using the random clone strategy followed by oligonucleotide‐directed sequencing. The segments contains five long open reading frames, YCR521, 522, 523, 524 and 526, with only short distances between them. YCR523 (333 codons) endodes a ribokinase, a new function for yeast. YCR526 originates inside the MAT cassette, which is in continuity with the present segment, and extends over 358 codons outside of MAT. YCR524 (923 codons) codes for a putative membrane protein. YCR521, 522 and 524, have each been disrupted by insertion of a URA3 cassette and are non‐essential genes. An active ARS element is located within YCR523 or its vicinity. Copyright © 1990 John Wiley & Sons Ltd.

引用

页码：521 / 534

页数：14

共 25 条

[1] IDENTIFICATION, PURIFICATION, AND CLONING OF A POLYPEPTIDE (PRTF/GRM) THAT BINDS TO MATING-SPECIFIC PROMOTER ELEMENTS IN YEAST [J].