AROMATASE-ACTIVITY IN HUMAN ADIPOSE-TISSUE STROMAL CELLS - EFFECT OF GROWTH-FACTORS

被引：30

作者：

LUEPRASITSAKUL, P ^{[1
]}

LATOUR, D ^{[1
]}

LONGCOPE, C ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS,SCH MED,DEPT OBSTET & GYNECOL,WORCESTER,MA 01655

来源：

STEROIDS | 1990年 / 55卷 / 12期

关键词：

STEROIDS; INSULIN; INSULIN-LIKE GROWTH FACTOR; AROMATASE ACTIVITY;

D O I：

10.1016/0039-128X(90)90049-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adipose tissue is a major, nonglandular site for the aromatization of androgens to estrogens. In this tissue, the aromatase activity resides primarily in the stromal cells, and we have used cultures of stromal cells to study the effects of insulin and insulin-like growth factor I (IGF-I) on aromatase activity. Adipose tissue, obtained during indicated surgery, was digested with collagenase, and the stromal cells were isolated and cultured. Aromatase activity was determined by measuring the tritiated water (H2O)-H-3 in the medium after incubating stromal cells with [1-beta-H-3]androstenedione. Insulin and IGF-I had no effect on the aromatase activity in cultured adipose stromal cells at concentrations of 10 to 1,000-mu-U/ml. However, insulin (100 to 1,000-mu-U/ml) and IGF-I (500 ng/ml) markedly attenuated the stimulatory effect of (Bu)2cAMP, but significantly augmented the dexamethasone-stimulated aromatase activitity. The greater effects of IGF-I compared with the effect of insulin are compatible with both effects being mediated through the IGF-I receptor. In addition, the effects of insulin in attenuating the aromatase activity in adipose tissue could potentiate its role in hyperandrogenic syndromes in women.

引用

页码：540 / 544

页数：5

共 23 条

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