BIODEGRADATION OF TRIIODOPHENOL BY CELL-FREE-EXTRACTS OF A PENTACHLOROPHENOL-DEGRADING FLAVOBACTERIUM SP

被引：17

作者：

XUN, L

ORSER, CS

机构：

[1] Department of Bacteriology, Biochemistry University of Idaho, Moscow

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90482-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pentachlorophenol (PCP) degrading Flavobacterium sp. ATCC 39723 was found to degrade other polyhalogenated phenolic compounds, including triiodophenol, tribromophenol, and trichlorophenol. Each Compound was able to induce the degradation of the other compounds. A PCP Flavobacterium sp. mutant, F-2, was unable to degrade any of the halogenated compounds. The results suggest that all of the polyhalogenated phenols were degraded by the same enzyme system. This observation led us to exploit the sensitive leuco crystal violet assay, which measures the iodide released from triiodophenol, Cell free extracts from PCP-induced cells were able to release lodide from triiodophenol. The reaction required NADPH and oxygen. © 1991.

引用

页码：43 / 48

页数：6

共 14 条

[1] RHODOCOCCUS-CHLOROPHENOLICUS SP-NOV, A CHLOROPHENOL-MINERALIZING ACTINOMYCETE [J].