CYTOKININS OF CULTURED CARROT CELLS

被引：2

作者：

SALEM, S ^{[1
]}

LINSTEDT, D ^{[1
]}

REINERT, J ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST PFLANZENPHYSIOL & ZELLBIOL,FACHBEREICH BIOL,D-1000 BERLIN 33,FED REP GER

来源：

PROTOPLASMA | 1979年 / 101卷 / 1-2期

关键词：

Cytokinins; Daucus carota L; Plant tissue culture;

D O I：

10.1007/BF01293439

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cytokinin-autotrophic strains of carrot callus contained active substances with Chromatographic mobilities on Sephadex LH-20 corresponding to 6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine (Zeatin, Z), 6-(3-methylbut-2-enylamino)purine (iP) and the ribosides (9R)Z and (9R)iP. The apparent major activity was found in a fraction, with an elution volume of 242-291 ml. Hydrolysis of this fraction with HCl and Β-Glucosidase gave rise to Z, indicating that the major active compound is a polar conjugate of Zeatin. In all experiments the extracts were tested immediately after preparation; deep freeze storage, led to a considerable loss of activity in polar fractions. while the free base cytokinins and their ribosides showed increased activity levels. Analogous results were obtained by means of paper chromatography. © 1979 Springer-Verlag.

引用

页码：103 / 109

页数：7

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