ENKEPHALIN INDUCES CA-2+ MOBILIZATION IN SINGLE CELLS OF BRADYKININ-SENSITIZED DIFFERENTIATED NEUROBLASTOMA HYBRIDOMA (NG108-15) CELLS

被引：22

作者：

TOMURA, H

OKAJIMA, F

KONDO, Y

机构：

[1] Department of Physical Biochemistry, Institute of Endocrinology, Gunma University, Maebashi

来源：

NEUROSCIENCE LETTERS | 1992年 / 148卷 / 1-2期

关键词：

ENKEPHALIN; BRADYKININ; CYTOSOLIC CA-2+; PERTUSSIS TOXIN; NG108-15; CELL;

D O I：

10.1016/0304-3940(92)90812-L

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A study of the intracellular Ca2+ ([Ca2+]i) response of differentiated neuroblastoma x glioma hybrid cells (NG108-15 cell) to enkephalin (EK) was carried out by fura-2 video-imaging. EK alone did not influence [Ca2+]i in single cells. The opioid did, however, induce a marked [Ca2+]i rise, when the cells were incubated with bradykinin (BK) prior to the EK treatment. Such BK-assisted stimulation of the differentiated hybridoma cells by EK was completely abolished by pertussis toxin treatment. These results suggest that in single NG108-15 cells, EK induces Ca2+ mobilization which is assisted by cross-talk between the EK and BK receptor systems via a pertussis toxin-sensitive G protein.

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页码：93 / 96

页数：4

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