CLONING, SEQUENCE-ANALYSIS AND YEAST EXPRESSION OF THE EGL1 GENE FROM TRICHODERMA-LONGIBRACHIATUM

被引：33

作者：

GONZALEZ, R ^{[1
]}

RAMON, D ^{[1
]}

PEREZGONZALEZ, JA ^{[1
]}

机构：

[1] CSIC,INST AGROQUIM & TECNOL ALIMENTOS,UNIDAD BIOINGN,JAIME ROIG 11,E-46010 VALENCIA,SPAIN

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1992年 / 38卷 / 03期

关键词：

D O I：

10.1007/BF00170088

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A gene (egl1) encoding an endoglucanase (EGL1) from Trichoderma longibrachiatum has been cloned and sequenced. This gene, homologous to the T. reesei egl1 gene, differs from it in the length of the introns (particularly the first one) and encoded protein. A cDNA fragment obtained by the rapid amplification of cDNA ends method, which takes advantage of the polymerase chain reaction, has been expressed in yeast under control of the cyc-gal inducible promoter and yeast clones able to secrete active enzyme have been obtained.

引用

页码：370 / 375

页数：6

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