Use of "AnyDirect PCR buffer'' for PCR amplification of washed bloodstains: A case report

被引：6

作者：

Barbaro, A. ^{[1
,3
]}

Cormaci, P. ^{[1
]}

Teatino, A. ^{[2
]}

Barbaro, A. ^{[1
,3
]}

机构：

[1] SIMEF, Dept Forens Genet, I-89128 Reggio Di Calabria, Italy

[2] SIMEF, Dept Chem, I-89128 Reggio Di Calabria, Italy

[3] SIMEF, I-89128 Reggio Di Calabria, Italy

来源：

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2008年 / 1卷 / 01期

关键词：

Washed bloodstains; Direct PCR; MiniSTRs;

D O I：

10.1016/j.fsigss.2007.10.201

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

AnyDirect PCR buffer (BioQuest) permits to perform direct PCR from different kinds of forensic samples (blood, saliva, sperm, etc.) without any DNA purification step. This is very useful in particular when working with small quantity of sample since it avoids the risk of loosing sample step by step as often happens with traditional DNA extraction procedures. In the present casework we analyzed some bloodstains found by luminol test onto washed clothes and linen: all samples were analyzed either using the AmpFlSTRs Identifiler kit (Applied Biosystems). (Cs) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：11 / 12

页数：2