DIFFERENTIAL MOLECULAR-WEIGHT DISTRIBUTIONS IN HIGH-PERFORMANCE SIZE-EXCLUSION CHROMATOGRAPHY

被引:130
作者
SHORTT, DW
机构
[1] Wyatt Technology Corporation, Santa Barbaro, California 93103
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 16期
关键词
D O I
10.1080/10826079308019695
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The definition and derivation of differential molecular weight distribution functions are thoroughly discussed. Common errors appearing in the literature are revealed and corrected. For high performance size exclusion chromatography (HPSEC), the importance of the calibration curve and its slope is discussed, and the application to gel permeation chromatography (GPC) is illustrated with simulations and real data.
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页码:3371 / 3391
页数:21
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